The steel strand cables anchored on the anchorage saddle on the tower in cable-stayed bridges have anti-sliding anchoring properties due to the frictional effect inside the metal splitting tubes. This study analyzes the transmission mechanism of the overall anti-sliding anchoring structure of the cables by implementing single-side arrangement of anti-sliding keys or dual-directional crossing arrangement of anti-sliding keys. Additionally, the principles and construction techniques of using ultra-tensile negative friction lock-in anti-sliding keys for cable-stayed bridges are proposed. Considering the possibility of deformation or blockage of individual splitting tubes during construction, which may prevent the steel strands from passing through and result in quality accidents, preventive measures are necessary. Adding spare conduits within the existing splitting tubes is a simple and effective method, which can also serve as backup pipes for future bridge reinforcement needs.