By combining finite element analysis with cable tower model test, the stress analysis of the tower anchorage area of the low pylon cable-stayed bridge in Sanguan Hanjiang Highway, Wuhan City, was carried out to verify the static load and anti-slip performance of the single-sided bidirectional anti-slip device of the cable tower anchorage zone. The cable replacement process of a single steel strand in the operation stage of the real bridge was simulated by test, which provided technical support for design and construction. The test results show that the structure of the cable pylon anchorage area of the bridge is safe under stress, the anti-slip structure of the cable pylon anchorage area and the cable changing process of a single steel strand meet the design and specification requirements.