The fatigue properties of corroded reinforced concrete beams reinforced with non-prestressed arylon fabric (AFRP) and prestressed AFRP with permanent anchorage are studied. Through fatigue test, the influence of prestress level and steel corrosion degree on fatigue failure mechanism, fatigue life and deflection of reinforced beams is discussed. The test results show that the fatigue failure mechanism of prestressed AFRP and non-prestressed reinforced beams is the same, and both are fatigue fracture of longitudinal reinforcement. The fatigue resistance of prestressed AFRP reinforced corroded concrete beams is obviously better than that of non-prestressed reinforced beams. The higher the prestress level, the higher the fatigue life of moderately corroded beams after reinforcement; The higher the corrosion rate, the lower the fatigue life of the beam, and the reduction of fatigue life of the severely corroded beam is more obvious than that of the moderately corroded beam. The equivalent fatigue notch coefficient Kf of the corroded longitudinal bar is obtained by comparing it with the plain steel bar. The results show that the fatigue life of corroded beams decreases obviously with the increase of steel bar corrosion rate. Based on the test results, a formula for calculating fatigue life of moderately corroded reinforced concrete beams reinforced by prestressed AFRP is established, which can be used as a reference for bridge reinforcement design.