Generally, epoxy gripping type is used for overall anti-slip of cable in cable-stayed Bridges with low towers, but this device cannot realize anti-slip of single strand and single cable change. The cross-key anti-slip device is proposed to study its application in low tower cable-stayed Bridges. Through finite element analysis, anti-slip force test, fatigue life and layout study, it can be seen that the anti-slip key can reliably hold the steel strand, has sufficient anti-slip ability and good anti-fatigue property, and adopts the cross-layout form to solve the problem of anti-slip and single cable replacement of a single steel strand. The tensioning process of the cross anti-slip key was summarized and perfected according to the simulated tensioning process test, and it was successfully applied in Fenhe Bridge in Linfen, Shanxi Province.