In order to meet the technical requirements of GB/T14370-2000 national standard and JGJ85-2002 industry standard, the analytical calculation and finite element analysis are carried out in the design and experimental study of the toothed single hole opening anchorage for prestressing tendons of steel strand. On this basis, through the design optimization, the reliability and applicability of the product are further improved, and the processing cost is reduced. The methods and results of relevant calculation and analysis are discussed in detail in this paper, which can provide reference for the development of similar products.